Recombinant Rat IL-7

Interleukin 7 (IL-7) is a glycoprotein cytokine initially referred to as pre-B cell growth factor because of its growth-promoting activity for cells early in the B lymphocyte lineage. Subsequent to IL-7 gene cloning and expression, IL-7 was also found to influence the activity of cells in the T lineage.¹ IL-7 stimulates the differentiation of multipotent (pluripotent) hematopoietic stem cells into lymphoid progenitor cells. It also stimulates proliferation of all cells in the lymphoid lineage (B and T lymphocytes and NK cells). IL-7 is not only a potent grown stimulus for pre-B cells, but also plays a role in T cell activation.² IL-7 has significant clinical and biomedical potential, such as cancer therapy and HIV infections.³

The biological activity of Rat Interleukin-7 is determined by the dose-dependent stimulation of the proliferation of mouse 2E8 cells. The expected ED₅₀ for this effect is 5 x 10⁶ units/mg.

MDCHIKDKDG KAFGSVLMIS INQLDKMTGT DSDCPNNEPN FFKKHLCDDT KEAAFLNRAA RKLRQFLKMN ISEEFNDHLL RVSDGTQTLV NCTSKEEKTI KEQKKNDPCF LKRLLREIKT CWNKILKGSI

Lyophilized

The molecular weight of Recombinant Rat IL-7 is Mr 15 kDa.

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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1. Namen, AE. et al. (1990) Int J Cell Cloning 1: 168
2. Reed, SG. et al. (1989) J Exp Med. 169: 707
3. Prakash, S. et al. (2008) Appl. of Biochem Biotechnol. 151: 93