Recombinant Rat SCF

Stem cell factor (SCF), otherwise known as KIT ligand or Steel factor¹ is a paracrine regulator of germ cell development.² SCF has the ability to stimulate the growth of hematopoietic progenitor cells and to generate colony-forming cells (CFC) from highly enriched populations of hematopoietic cells.³ SCF is produced by the Sertoli cells and involved in the local regulation of spermatogenesis⁴ and liver regeneration.⁵ SCF in conjunction with interleukin-31 may play a significant role in airway remodeling by promoting the recruitment of bone marrow-derived fibroblast precursors into the lung with the capacity to promote lung myofibroblast differentiation.⁶

The biological activity of Rat Stem Cell Factor is determined by the dose-dependent stimulation of the proliferation of the human MO7e cell line. The expected ED₅₀ for this effect is 1 x 10⁵ units/mg.

MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

Lyophilized

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

1. Toppari, J. et al. (1999) Endocrin 140: 1492
2. Adamson, JW. et al. (1991) PNAS 88: 7420
3. Hervé, L. et al. (2005) J of Endocrin. 186: 131
4. Ramadori, G. et al. (2009) Lab Invest. 89(5):562-74.
6. Phan, SH. et al. (2009) AM J Pathol. 174: 390