Streptavidin – FITC
Data
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BackgroundStreptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (Kd~10
-15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin. Protein DetailsFormat Fluorescein (FITC) Product Concentration 0.2 mg/ml Formulation This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Working Concentration Streptavidin-FITC has been tested by flow cytometric analysis to detect biotinylated primary antibodies. A woring dilution ≤ 0.125 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume) is suitable for most applications.
Storage and Stability This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day Ambient Certificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.