Streptavidin – HRP

Streptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (K_d~10 ^-15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin.

This HRP-conjugated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4, 1% BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)

This StreptAvidin - HRP conjugate has been covalently conjugated to Horseradish Peroxidase. The conjugate is then purified chromatographically to remove the unconjugated enzyme and StreptAvidin

Western blotting: 1:5,000 - 1:10,000
ELISA: 1:5,000 - 1:20,000
Immunohistochemistry: 1:100 - 1:1,000
Western blotting and ELISA Chemiluminescent: 1:20,000 - 1:100,000

However, each investigator should determine their own optimal working dilution for each specific research application.

Stable when stored at 2 - 8°C. Do not freeze

Next Day 2-8°C