UltraCoat™ ELISA Plate Coating Buffer 10X

Leinco's ELISA Plate Coating Solution-10X Concentrate is a ready-to-dilute solution optimized for your ELISA assays. This proprietary blend of monobasic and dibasic phosphates in physiological saline creates the ideal pH and ionic strength environment. Maximize the specific binding of your antigens or antibodies to your ELISA plates, regardless of your system. Streamline your ELISA workflow with Leinco and achieve superior results.

Store at 2-8°C

Next Day 2-8°C

Minimum of 3 years prior to dilution when stored at 2-8°C. In order to minimize the risk of contamination fresh working solution should be prepared daily

Maximize the success of your ELISA experiments with UltraCoat^TM, a proprietary coating buffer formulated to optimize antibody and antigen binding to polystyrene plates. By maintaining the ideal pH and ionic strength, UltraCoat^TM ensures superior protein immobilization and preserves their 3D structure for enhanced functionality. This translates to increased specific binding, reduced background noise, and ultimately, more accurate and reliable ELISA results. Elevate your research with Leinco's UltraCoat and unlock the full potential of your ELISA assays.

Increased Specific Signal and Decreased Background – UltraCoat™ ELISA Plate Coating Buffer has an optimal pH and ionic strength designed for increased adsorption and consistent plate coating to increase specific binding and reduce non-specific interference.
Save Valuable Coating Protein and Detection Conjugate – By stabilizing coated proteins and preserving their tertiary structures, the specific signal will be increased and you may find that you can use less protein to coat your plates and less detection conjugate to obtain optimal signal.
Ease of Use – Simply add 1 part UltraCoat™ ELISA Plate Coating Buffer 10X Stock Solution to 9 parts high purity deionized water. Following dilution, the buffer is ready to use.

Basic ELISA Protocol
1.) Prior to use, simply add 1 part UltraCoat^TM to 9 parts high purity deionized water. Note: Allow working solution to equilibrate to room temperature (25°C) prior to use.
2.) Add your antibody or antigen into enough coating buffer to treat the target surface by covering completely with solutionAssay Note: Coating volume can range from 50 – 300 µl/well). Appropriate coating concentrations will range from < 0.1 µg/ml to > 10 µg/ml and each investigator should determine their own optimal coating concentration for their specific application.
3.) Pipette the desired coating volume into the microwells (50 – 300 µl/well).
4.) For best results, allow plates to incubate with coating solution overnight at 4°C protected from light. Wrap the plate(s) in plastic wrap or incubate in a covered humidified chamber to minimize evaporation.
5.) Dump or aspirate the coating solution out of the wells and wash plates > 4 times to minimize background staining.
6.) Pipette an appropriate blocking buffer, such as UltraBlock-FISH™ ELISA Blocking Buffer “Ready Use” (Leinco Prod. No.: B396), onto the plate at a higher volume than the coating solution (200 – 300 µl/well).
7.) Allow plates to incubate with blocking buffer for 3 – 24 hours at room temperature (25°C) protected from light. Wrap the plate(s) in plastic wrap or incubate in a covered humidified chamber to minimize evaporation.
8.) Aspirate the blocking solution from the wells.
9.) The assay can be performed at this point or the plate(s) can be dried and sealed for later use.
10.) Dry the plate(s) loosely covered with aluminum foil on the bench top or in a drying oven at room temperature or warmer for 2 – 24 hours.
11.) Once plate(s) are dry, seal in an air-tight foil pouch with a desiccant and store at 2° - 8°C.

Leinco is a leading USA manufacturer, located in St. Louis, Missouri, of premium immunoassay reagents, committed to advancing disease diagnostics and treatment. Our ISO 13485-certified facility adheres to the highest quality standards, ensuring consistent and reliable performance for your immunoassay development. Our comprehensive suite of high-quality reagents empowers assay developers to build consistent, reliable, and sensitive immunoassays for a wide range of applications, including ELISA, point-of-care diagnostics, and beyond.