Roller Bottle Hybridoma Antibody Production

RB4: Your Cells’ Happy Place

What is the RB4 Standard Service?

Our hybridoma RB4 (4L) roller bottles production services use roller bottles, slowly rotated by a machine which offer a practical solution for expanding cell cultures in research and development settings. This rotation allows cells to adhere and grow across the entire inner surface, maximizing contact with the liquid media. When larger quantities of cells are needed, roller bottles become an attractive option due to their increased surface area compared to T-flasks, which are often used for initial cell culture establishment. As with T-flasks, the choice of coated or non-coated roller bottles depends on whether adherent or suspension cells are being cultured.

How it Works

Roller bottle process
PhaseDescriptionIncluded Optional Add-Ons
0. ReceiveInstructions for cell preperation and shipping your cellsX
1. Cell CultureAssessment of cell line activity and clonatility for productivity of IgG levelsX
1. Cell CultureMycoplasma testing Certification X
1. Cell CultureAntibody isotyping service X
2. Adaptation & Cell BankingCells will be adapted to grow in 5-10% low bovine IgG serum media (DMEM)X
2. Adaptation & Cell BankingStorage of cells at Leinco for rapid re-supply and risk midigation (ten vial hybridma bank)X
3. ProductionCell culture Scale-Up and ProductionX
3. ProductionExpansion of cells using optimized hybridoma roller bottle production protocolX
4. PurificationPurification of supernatant on Protein A or GX
4. PurificationBuffer exchange to Standard PBS or add a preservative/stabilizer if requiredX
4. PurificationSterile filtering of final productX
5. Vialing & QCTesting to confirm <1EU/mg endotoxin; In vivo GOLD Standard X
5. Vialing & QCSterile vialing of finished product. Custom aliquoting availableX
5. Vialing & QCStandard antibody quality control testingX
5. Vialing & QCCertificate of AnalysisX
6. Optional QCMurine pathogen screening service X
6. Optional QCHybridoma Sequencing X
6. Optional QCBinding Assay by ELISA, Western Blot or Flow Cytometry X
7. ShippingShipping on cold packs or Dry IceX

Standard & Optional Quality Control

Standard Quality Control TestSpecification
Purity by SDS Page (Reduced & Non-reduced)> 90%
Appearance of a Liquid Sample Prior to Final Storagee.g. clear, colorless, or opaque
pH Determination7.2 - 7.4
A280 Antibody Concentration Determination> 1.0 mg/mL
Formulation0.01 M Phosphate buffered Saline (PBS), 150 mM NaCl with no carrier protein, potassium, or preservatives added
Endotoxin Level by Gel Clot Method (LAL)< 1.0 EU/mg (GOLD Standard)
% Aggregates by Analytical SECFor information only
Optional Quality Control TestSpecification
Optional Isoelectric focusing (IEF) GelsFor information only
Optional Application Specific TestingELISA, Flow Cytometry, and/or Western Blot
Optional Murine pathogen IMPACT™ testingPass/Fail

Why Leinco is the Solution

Leinco stands out as a top choice for hybridoma roller bottle production due to our extensive experience and expertise in cell culture manufacturing. With a proven track record in producing high-quality antibodies and cell lines, Leinco ensures consistent and reliable results. Our state-of-the-art facilities adhere to strict quality control standards, guaranteeing optimal conditions for hybridoma growth and productivity.

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