Anti-Mouse IL-17 (Clone 50101)
Anti-Mouse IL-17 (Clone 50101)
Product No.: I-699
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Clone 50101 Target IL-17 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CTLA8, IL17A, Interleukin-17 Isotype IgG2a Applications ELISA Cap , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse IL-17 (>98%) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830820 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:I-775 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-533. Western Blotting: To detect Mouse IL-17 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-17 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Rat Anti-Mouse Interleukin 17 (IL-17) (Clone 50101) recognizes an epitope on Mouse IL-17. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows less than 25% cross-reactivity with rhIL-17 under non-reducing conditions. Background Interleukin 17 (IL-17) is a T cell cytokine that induces monocyte migration and inflammation.1 IL-17-producing cell could serve as a potential prognostic marker and a novel therapeutic target for hepatocellular carcinoma (HCC).2 IL-17 may be important in recruiting monocytes into the joints of patients with RA, supporting IL-17 as a therapeutic target in RA.1 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Pope, RM. et al. (2009) J Immunol. 182: 3884 2. Zheng, L. et al. (2009) J Hepatol. 50(5):980-989. Technical Protocols  Certificate of Analysis |
Related Products
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Prod No. | Description |
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I-655 | |
I-667 | |
I-699 | |
I-533 | |
I-770 | |
I-272 | |
I-775 |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
