Anti-Human CCL22-Biotin
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Antibody DetailsProduct DetailsReactive Species Human Host Species Chicken Immunogen Purified Recombinant Human CCL22 (>98%) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831284 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human CCL22 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL22 is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M171. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/mL when used in conjunction with PN:M1092 as the capture antibody at 2 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Human CCL22. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Chicken Anti-Human Chemokine (C-C Motif) Ligand 22 (CCL22) recognizes Human CCL22. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Chemokine (C-C motif) ligand 22, also known as CCL22 is secreted by dendritic cells and macrophages, and elicits its effects on its target cells by interacting with cell surface chemokine receptors such as CCR4.1 CCL22 selectively chemo-attract Th2 cytokine-producing cells2,3 and acts as an important activator of eosinophils once these cells have migrated into tissue.4 PubMed NCBI Gene Bank ID UniProt.org Research Area Chemokine References & Citations1. Sozzani, S. et al. (2001) Eur. J. Immunol. 31: 812 2. Elias, CG. et al. (1998) J Immunol. 161: 5027 3. Gray, PW. et al. (1997) J Exp Med. 185: 1604 4. Teixeira, MM. et al. (2003) J Leuko Biol. 73: 356 Technical ProtocolsELISA Det  Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
