Anti-Mouse TNFRSF19

Anti-Mouse TNFRSF19

Product No.: T475


Target TNFRSF19 Product Type Polyclonal Antibody Alternate Names Tumor Necrosis Factor Receptor Superfamily, Member 19, TNFRSF19, Toxicity and JNK Inducer (TAJ), TAJ-Alpha, TRADE, TROY Applications ELISA Cap , IHC FFPE , WB


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Antibody Details Product Details Reactive Species Mouse Host Species Goat Immunogen Purified Recombinant Mouse TNFRSF19 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831975 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.2-0.8 µg/mL. A suitable detection antibody is PN:T502 at a concentration of approximately 100 µg/ml. A suggested standard for this assay is PN:T407. Western Blotting: To detect Mouse TNFRSF19 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse TNFRSF19 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T407. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5 - 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Goat Anti-Mouse Tumor Necrosis Factor Receptor Superfamily, Member 19 (TNFRSF19) recognizes Mouse TNFRSF19. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Tumor necrosis factor receptor superfamily, member 19, also known as TNFRSF19 and TROY is a novel murine tumor necrosis factor receptor.¹ It can be detected in most murine tissues examined, particularly in brain, reproductive organs, and late developmental stages of murine embryo, but not in tissues of the immune system.¹ TNFRSF19 is a member of the TNF-receptor superfamily and is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. PubMed TNFRSF19 NCBI Gene Bank ID 29820 UniProt.org More Information on Uniprot.org References & Citations 1. Chen, L. et al. (1999) Genomics 62: 103 View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols IHC FFPE Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Goat

Immunogen

Purified Recombinant Mouse TNFRSF19 (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831975

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.2-0.8 µg/mL. A suitable detection antibody is PN:T502 at a concentration of approximately 100 µg/ml. A suggested standard for this assay is PN:T407.
Western Blotting: To detect Mouse TNFRSF19 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse TNFRSF19 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:T407.

Additional Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5 - 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Mouse Tumor Necrosis Factor Receptor Superfamily, Member 19 (TNFRSF19) recognizes Mouse TNFRSF19. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Tumor necrosis factor receptor superfamily, member 19, also known as TNFRSF19 and TROY is a novel murine tumor necrosis factor receptor.¹ It can be detected in most murine tissues examined, particularly in brain, reproductive organs, and late developmental stages of murine embryo, but not in tissues of the immune system.¹ TNFRSF19 is a member of the TNF-receptor superfamily and is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development.

PubMed

TNFRSF19

NCBI Gene Bank ID

29820

UniProt.org

More Information on Uniprot.org